4X0J
Trypanosoma brucei haptoglobin-haemoglobin receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.900, 47.790, 203.380 |
| Unit cell angles | 90.00, 92.79, 90.00 |
Refinement procedure
| Resolution | 203.140 - 1.850 |
| R-factor | 0.20074 |
| Rwork | 0.199 |
| R-free | 0.23998 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4X0I |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.658 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 203.140 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.099 | 0.640 |
| Number of reflections | 44672 | |
| <I/σ(I)> | 10.2 | |
| Completeness [%] | 97.4 | 96.4 |
| Redundancy | 3.1 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.15 M KBr, 30 % w/v PEG 2000 MME |
