4X07
Crystal structure of P domain from norovirus strain Saga4 in complex with HBGA type A (triglycan)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-28 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97246 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 97.960, 58.640, 114.590 |
| Unit cell angles | 90.00, 105.49, 90.00 |
Refinement procedure
| Resolution | 43.183 - 1.460 |
| R-factor | 0.1667 |
| Rwork | 0.165 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4oox |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.204 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.3) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.010 | 1.320 | |
| High resolution limit [Å] | 1.280 | 5.730 | 1.280 |
| Rmerge | 0.064 | 0.049 | 0.419 |
| Rmeas | 0.071 | 0.055 | 0.501 |
| Total number of observations | 708898 | ||
| Number of reflections | 157018 | 1866 | 10960 |
| <I/σ(I)> | 7.42 | 13.06 | 1.96 |
| Completeness [%] | 98.0 | 98.9 | 92.7 |
| Redundancy | 4.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 291 | 3M Sodium acetate |
