4X06
Crystal structure of P domain from norovirus strain Saga4 in complex with HBGA type B (triglycan)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97248 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.830, 58.650, 97.310 |
| Unit cell angles | 90.00, 107.34, 90.00 |
Refinement procedure
| Resolution | 32.323 - 1.218 |
| R-factor | 0.1354 |
| Rwork | 0.134 |
| R-free | 0.16120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.357 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.3) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 1.250 | ||
| High resolution limit [Å] | 1.220 | 5.450 | 1.220 |
| Rmerge | 0.035 | 0.031 | 0.354 |
| Rmeas | 0.043 | 0.039 | 0.442 |
| Total number of observations | 457076 | ||
| Number of reflections | 176472 | 2040 | 12148 |
| <I/σ(I)> | 13.92 | 34.3 | 2.54 |
| Completeness [%] | 96.7 | 94.7 | 90.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 291 | Sodium acetate |
