4X01
S. pombe Ctp1 tetramerization domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2012-06-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.071, 156.677, 48.927 |
| Unit cell angles | 90.00, 118.55, 90.00 |
Refinement procedure
| Resolution | 40.676 - 2.201 |
| R-factor | 0.2004 |
| Rwork | 0.198 |
| R-free | 0.24550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 1T6F |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.952 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 32678 |
| <I/σ(I)> | 20.7 |
| Completeness [%] | 99.9 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Precipitant: 0.2M sodium acetate, 0.1M Tris pH 8.5 16% PEG 4000, with 0.4 uL of 0.1M barium chloride dihydrate additive. Cryoprotection in 25% ethylene glycol. |
