4WZP
Ser65 phosphorylated ubiquitin, major conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.563, 53.285, 56.866 |
| Unit cell angles | 96.86, 104.94, 110.96 |
Refinement procedure
| Resolution | 32.280 - 1.900 |
| R-factor | 0.1956 |
| Rwork | 0.193 |
| R-free | 0.23750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ubq |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.997 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.280 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.124 | 0.425 |
| Number of reflections | 39104 | |
| <I/σ(I)> | 4.6 | 2.1 |
| Completeness [%] | 97.3 | 96.3 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 30% (w/v) PEG 8000, 0.2 M ammonium sulfate |
