Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-03-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 210.200, 448.800, 621.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 153.410 - 3.300 |
| Rwork | 0.191 |
| R-free | 0.25780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3TVE 3TVF |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 300.000 | 3.400 | |
| High resolution limit [Å] | 3.300 | 100.000 | 3.300 |
| Rmerge | 0.459 | 0.071 | 3.698 |
| Rmeas | 0.465 | 0.079 | 3.795 |
| Total number of observations | 35911012 | ||
| Number of reflections | 871092 | 13 | 74101 |
| <I/σ(I)> | 9.41 | 18.48 | 0.99 |
| Completeness [%] | 99.9 | 28.3 | 99.9 |
| Redundancy | 41.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | Tris-acetate, KSCN, PEG 550MME, PEG 20K |
