4WZJ
Spliceosomal U4 snRNP core domain
This is a non-PDB format compatible entry.
Replaces: 4V5UReplaces: 2Y9AReplaces: 2Y9BReplaces: 2Y9CReplaces: 2Y9DExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-07-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 31 |
| Unit cell lengths | 248.010, 248.010, 251.940 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 66.154 - 3.600 |
| R-factor | 0.17899 |
| Rwork | 0.177 |
| R-free | 0.22380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pjo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.399 |
| Data reduction software | XDS |
| Data scaling software | SCALA (6.0) |
| Phasing software | PHASER (2.5.2) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.160 | 3.680 |
| High resolution limit [Å] | 3.590 | 3.590 |
| Rmerge | 0.210 | 0.730 |
| Number of reflections | 202467 | |
| <I/σ(I)> | 3.3 | 1.1 |
| Completeness [%] | 82.9 | 20.8 |
| Redundancy | 2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 4-10% PEG 550 MME, 0.1M KSCN or (NH4)2SO4, 0.1 M TRIS PH 8.0-8.5 10 MM MGCL2, 2 MM CYCLEN. |
