4WZG
Structure of human ATG101
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-06-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.90000 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 40.150, 42.620, 67.210 |
Unit cell angles | 90.00, 97.31, 90.00 |
Refinement procedure
Resolution | 42.620 - 1.900 |
R-factor | 0.199 |
Rwork | 0.198 |
R-free | 0.22000 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.625 |
Data reduction software | XDS (January 10, 2014) |
Data scaling software | XSCALE |
Phasing software | SHELXDE |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 42.620 | 2.000 | |
High resolution limit [Å] | 1.900 | 8.500 | 1.900 |
Rmerge | 0.039 | 0.028 | 0.528 |
Rmeas | 0.045 | 0.033 | 0.528 |
Total number of observations | 56530 | ||
Number of reflections | 13298 | 219 | 221 |
<I/σ(I)> | 16.62 | 37.25 | 3.1 |
Completeness [%] | 73.9 | 99.1 | 22.8 |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | PEG3350, MES, NACL, BME |