4WZG

Structure of human ATG101

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-06-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.90000
Spacegroup name	P 1 2 1
Unit cell lengths	40.150, 42.620, 67.210
Unit cell angles	90.00, 97.31, 90.00

Refinement procedure

Resolution	42.620 - 1.900
R-factor	0.199
Rwork	0.198
R-free	0.22000
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.625
Data reduction software	XDS (January 10, 2014)
Data scaling software	XSCALE
Phasing software	SHELXDE
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.620		2.000
High resolution limit [Å]	1.900	8.500	1.900
Rmerge	0.039	0.028	0.528
Rmeas	0.045	0.033	0.528
Total number of observations	56530
Number of reflections	13298	219	221
<I/σ(I)>	16.62	37.25	3.1
Completeness [%]	73.9	99.1	22.8
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	PEG3350, MES, NACL, BME