4WZF

Crystal structural basis for Rv0315, an immunostimulatory antigen and pseudo beta-1, 3-glucanase of Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-05-16
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97917
Spacegroup name	C 1 2 1
Unit cell lengths	135.133, 65.847, 67.449
Unit cell angles	90.00, 105.17, 90.00

Refinement procedure

Resolution	24.783 - 1.699
R-factor	0.1831
Rwork	0.182
R-free	0.21120
RMSD bond length	0.008
RMSD bond angle	1.171
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.699	1.699
Rmerge	0.136	0.228
Number of reflections	62875
<I/σ(I)>	9.51	7.13
Completeness [%]	98.4	99.9
Redundancy	4	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.2	293	0.2M Ammonium phosphate monobasic, 20% PEG 3350