4WZE
Crystal structure of P domain from norovirus strain Saga4 in complex with HBGA type Ley (tetraglycan)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-11-28 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97250 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 97.020, 58.500, 113.860 |
| Unit cell angles | 90.00, 108.10, 90.00 |
Refinement procedure
| Resolution | 31.759 - 1.460 |
| R-factor | 0.1791 |
| Rwork | 0.177 |
| R-free | 0.21170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4oox |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.5.3) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.160 | 1.490 | |
| High resolution limit [Å] | 1.450 | 6.490 | 1.450 |
| Rmerge | 0.028 | 0.021 | 0.455 |
| Rmeas | 0.035 | 0.027 | 0.582 |
| Total number of observations | 234946 | ||
| Number of reflections | 102227 | 1204 | 7436 |
| <I/σ(I)> | 10.16 | 23.28 | 1.8 |
| Completeness [%] | 95.1 | 94.1 | 93.9 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.9 | 291 | Sodium acetate |
