4WZ7
Crystal structure of mitochondrial NADH:ubiquinone oxidoreductase from Yarrowia lipolytica.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 5 |
Detector technology | PIXEL |
Collection date | 2012-02-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0000 |
Spacegroup name | H 3 2 |
Unit cell lengths | 317.740, 317.740, 818.970 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 3.600 |
R-factor | 0.3168 |
Rwork | 0.316 |
R-free | 0.34140 |
Structure solution method | MIR |
RMSD bond length | 0.011 |
RMSD bond angle | 1.500 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | SHARP |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.660 |
High resolution limit [Å] | 3.600 | 3.600 |
Rmerge | 0.518 | 5.940 |
Number of reflections | 183009 | |
<I/σ(I)> | 8.9 | |
Completeness [%] | 99.0 | 100 |
Redundancy | 50.6 | 32.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 291 | PEG 3350, calcium acetate, glycerol |