4WZ2
Crystal structure of U-box 2 of LubX / LegU2 / Lpp2887 from Legionella pneumophila str. Paris, Ile175Met mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9790433 |
| Spacegroup name | P 4 3 2 |
| Unit cell lengths | 160.033, 160.033, 160.033 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.720 - 3.408 |
| R-factor | 0.1813 |
| Rwork | 0.177 |
| R-free | 0.21560 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.535 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.460 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.126 | 0.649 |
| Number of reflections | 9956 | |
| <I/σ(I)> | 14.21 | 2.97 |
| Completeness [%] | 98.6 | 100 |
| Redundancy | 6.5 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 15 mg/ml protein, 1.6 M ammonium sulfate, 0.1 M HEPES (pH 7.5) and 2% hexanediol |
