4WYJ
Adenovirus 3 head domain mutant V239D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-17 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 137.566, 137.566, 108.939 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.410 - 2.650 |
| R-factor | 0.1772 |
| Rwork | 0.176 |
| R-free | 0.20490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h7z |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.410 | 2.740 |
| High resolution limit [Å] | 2.650 | 2.650 |
| Number of reflections | 30619 | |
| <I/σ(I)> | 18.9 | 1.43 |
| Completeness [%] | 99.0 | 90.3 |
| Redundancy | 8.9 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 288 | 1.75M magnesium sulfate, 0.1M TAPS, pH 9.0 |
