4WYI
The crystal structure of Arabidopsis thaliana galactolipid synthase, MGD1 (apo-form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-05-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9811 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.610, 65.619, 80.401 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.810 - 2.500 |
R-factor | 0.1815 |
Rwork | 0.181 |
R-free | 0.19990 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.023 |
RMSD bond angle | 1.413 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.530 | 2.640 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 11358 | |
<I/σ(I)> | 14.3 | 4.1 |
Completeness [%] | 99.5 | 99.5 |
Redundancy | 4.8 | 4.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M magnesium chloride, 22% PEG 3350 |