4WYH
Crystal structure of PriX from the hyperthermophilic archaeon Sulfolobus solfataricus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ELECTRON MICROSCOPE |
Source details | RIGAKU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-20 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 28.258, 36.232, 83.758 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.424 - 1.950 |
R-factor | 0.1598 |
Rwork | 0.158 |
R-free | 0.18010 |
Structure solution method | SAD |
RMSD bond length | 0.019 |
RMSD bond angle | 1.733 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-2000 |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 23.424 |
High resolution limit [Å] | 1.950 |
Number of reflections | 6218 |
<I/σ(I)> | 105.56 |
Completeness [%] | 92.8 |
Redundancy | 11.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 289 | 0.2 M potassium iodide, 20% (w/v) polyethylene glycol 3,350, pH 7.0 |