4WYB

Structure of the Bud6 flank domain in complex with actin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-12-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 32
Unit cell lengths	138.753, 138.753, 356.651
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.418 - 3.493
R-factor	0.2134
Rwork	0.212
R-free	0.25810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mn7
RMSD bond length	0.005
RMSD bond angle	1.080
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	45.418
High resolution limit [Å]	3.493
Rmerge	0.180
Number of reflections	97910
<I/σ(I)>	4.4
Completeness [%]	99.8
Redundancy	8.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	3.5M sodium formate, 0.1M CaCl2, and 5 mM TCEP