4WY9

Crystal structure of the periplasmic sensory domain of the Campylobacter jejuni chemoreceptor Tlp1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-09-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	39.260, 43.320, 50.900
Unit cell angles	92.52, 111.38, 114.73

Refinement procedure

Resolution	15.000 - 1.400
R-factor	0.1187
Rwork	0.117
R-free	0.15420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	TlpC
RMSD bond length	0.014
RMSD bond angle	1.308
Data scaling software	Aimless (0.3.11)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.270	1.420
High resolution limit [Å]	1.400	1.400
Rmerge	0.031	0.149
Rpim	0.031	0.149
Total number of observations	97980	3448
Number of reflections	49677
<I/σ(I)>	11.9	3.9
Completeness [%]	91.5	66.2
Redundancy	2	2
CC(1/2)	0.998	0.944

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	200 mM ammonium acetate, 23%(w/v) PEG 3350 and 100 mM Bis-Tris pH 5.5