4WXU
Crystal Structure of the Selenomthionine Incorporated Myocilin Olfactomedin Domain E396D Variant.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2013-10-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.484, 50.560, 50.537 |
Unit cell angles | 90.00, 97.27, 90.00 |
Refinement procedure
Resolution | 19.598 - 2.092 |
R-factor | 0.1852 |
Rwork | 0.178 |
R-free | 0.20140 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.830 |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.600 |
High resolution limit [Å] | 2.092 |
Number of reflections | 14363 |
<I/σ(I)> | 25.23 |
Completeness [%] | 97.2 |
Redundancy | 13.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289.15 | PEG 3350 Bis-Tris pH 6 magnesium formate |