4WXL
Crystal structure of a peptide deformylase from Haemophilus influenzae complex with Actinonin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-05-19 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.201, 79.850, 98.168 |
| Unit cell angles | 90.00, 97.99, 90.00 |
Refinement procedure
| Resolution | 26.690 - 2.330 |
| R-factor | 0.1755 |
| Rwork | 0.172 |
| R-free | 0.24147 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.676 |
| Data reduction software | HKL-3000 |
| Data scaling software | DENZO |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 26.690 |
| High resolution limit [Å] | 2.330 |
| Number of reflections | 27056 |
| <I/σ(I)> | 7.16 |
| Completeness [%] | 99.6 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 297 | 20-25% PEG 3350, 0.1M HEPES pH 7.0, 2% glycerol |
