4WXK
Crystal structure of a peptide deformylase from Haemophilus influenzae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-05-25 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.036, 80.961, 98.586 |
| Unit cell angles | 90.00, 97.81, 90.00 |
Refinement procedure
| Resolution | 25.370 - 2.050 |
| R-factor | 0.16382 |
| Rwork | 0.161 |
| R-free | 0.22161 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g2a |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.828 |
| Data reduction software | HKL-3000 |
| Data scaling software | DENZO |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.370 |
| High resolution limit [Å] | 2.050 |
| Number of reflections | 39520 |
| <I/σ(I)> | 11.67 |
| Completeness [%] | 97.7 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 300 | 20-25% PEG 3350, 0.1M HEPES pH 7.0, 2% glycerol |
