4WXJ

Drosophila muscle GluRIIB complex with glutamate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-15
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	52.314, 94.527, 119.538
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.054 - 2.002
R-factor	0.1993
Rwork	0.198
R-free	0.22950
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1s50
RMSD bond length	0.003
RMSD bond angle	0.647
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1819))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.069	0.840
Number of reflections	40664
<I/σ(I)>	27.6	2.3
Completeness [%]	99.9	99.5
Redundancy	6.5	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	Reservoir: 18% PEG 2K MME, 0.1 M Tris pH 8.0, 5 mM NiCl2 Protein buffer: 150 mM NaCl, 10 mM Tris pH 8.0, 2 mM Na glutamate, 1 mM EDTA multiple rounds of seeding