4WXB
Crystal Structure of Serine Hydroxymethyltransferase from Streptococcus thermophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2007-04-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.970 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 202.104, 113.431, 133.388 |
Unit cell angles | 90.00, 93.85, 90.00 |
Refinement procedure
Resolution | 66.540 - 2.050 |
R-factor | 0.193 |
Rwork | 0.191 |
R-free | 0.22100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1kkj |
RMSD bond length | 0.019 |
RMSD bond angle | 1.972 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.100 | 2.150 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.101 | 0.514 |
Number of reflections | 187621 | 15884 |
<I/σ(I)> | 11.1 | 2 |
Completeness [%] | 98.8 | 99.7 |
Redundancy | 8 | 1.96 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | HEPES 20 mM, pH 7,5 Sodium citrate 0.8-1.2 M Cacodylate 1 M, pH 6,5 |