4WW9

Crystal structure of binary complex Bud32-Cgi121 in complex with ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-11-14
Detector	PSI PILATUS 6M
Wavelength(s)	0.97911
Spacegroup name	I 41
Unit cell lengths	113.010, 113.010, 86.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.737 - 1.951
R-factor	0.1805
Rwork	0.179
R-free	0.20920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Methanocaldococcus jannaschii Bud32/Cgi121
RMSD bond length	0.005
RMSD bond angle	0.991
Data reduction software	XDS (2012)
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.740	2.021
High resolution limit [Å]	1.951	1.951
Number of reflections	39488
<I/σ(I)>	25.08
Completeness [%]	99.9	98.96
Redundancy	7.34

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	0.1 M Sodium Acetate pH 4.8, 2.0 M Ammonium Sulfate, 0.1 M NaCl, 10 mM Tris-HcL pH 7.5 and 5 mM ATP