4WW0

Truncated FtsH from A. aeolicus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2008-07-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	I 2 2 2
Unit cell lengths	138.230, 162.260, 170.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.010 - 2.960
R-factor	0.2107
Rwork	0.209
R-free	0.24840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2di4 2ce7
RMSD bond length	0.009
RMSD bond angle	1.120
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.030
High resolution limit [Å]	2.960	2.960
Rmerge	0.089	1.370
Number of reflections	39488
<I/σ(I)>	14.1	1
Completeness [%]	97.9	91.3
Redundancy	5.2	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	60 % Tacsimate pH 7.0, 10 mM AMP-PNP