4WVY

Double-heterohexameric rings of full-length Rvb1(ATP)/Rvb2(apo)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-07-23
Detector	PSI PILATUS 6M
Wavelength(s)	0.91889
Spacegroup name	H 3 2
Unit cell lengths	210.348, 210.348, 137.140
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.690 - 3.640
R-factor	0.2229
Rwork	0.221
R-free	0.25060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2c9o
RMSD bond length	0.008
RMSD bond angle	1.000
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.21)
Phasing software	PHASER
Refinement software	BUSTER-TNT

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	109.748	33.694	3.840
High resolution limit [Å]	3.640	11.510	3.640
Rmerge		0.073
Rmeas	0.133
Rpim	0.035	0.021	0.287
Total number of observations	190173	5701	26988
Number of reflections	13158
<I/σ(I)>	15.9	32.7	3.9
Completeness [%]	99.9	96	100
Redundancy	14.5	13.3	14.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	1 M sodium malonate pH 6.0