4WVX
Crystal structure of a phosphotriesterase-like lactonase Gkap in native form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-06-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97916 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 51.398, 89.380, 89.915 |
Unit cell angles | 90.00, 99.33, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.900 |
R-factor | 0.16757 |
Rwork | 0.166 |
R-free | 0.19200 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.474 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.900 |
Number of reflections | 63053 |
<I/σ(I)> | 16.9 |
Completeness [%] | 99.8 |
Redundancy | 7.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 287 | 14% PEG 20000, 0.1M MES pH6.5 |