4WVV
Chicken Galectin-8 N-terminal domain complexed with lactose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-28 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00003 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 56.459, 56.459, 201.239 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.063 - 1.205 |
| R-factor | 0.1529 |
| Rwork | 0.152 |
| R-free | 0.16580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yv8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.345 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.063 | 1.270 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.174 | 0.586 |
| Number of reflections | 59854 | |
| <I/σ(I)> | 15.2 | 7.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20 | 30.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 25% w/v PEG 1000, 100 mM HEPES Sodium salt pH 7.5 |
