4WVU
CRYSTAL STRUCTURE OF XIAP-BIR2 DOMAIN COMPLEXED WITH LIGAND BOUND
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-18 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 28.261, 39.328, 33.058 |
| Unit cell angles | 90.00, 95.51, 90.00 |
Refinement procedure
| Resolution | 25.240 - 2.020 |
| R-factor | 0.1411 |
| Rwork | 0.139 |
| R-free | 0.17810 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.870 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.330 | 2.130 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.128 | |
| Number of reflections | 4708 | |
| <I/σ(I)> | 18.9 | 8.6 |
| Completeness [%] | 97.4 | 84 |
| Redundancy | 3.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | .98 M AMMONIUM SULFATE and .1 M AMMONIUM FORMATE. Grew single crystals after streak seeding. |
