4WVR

Crystal structure of Dscam1 Ig7 domain, isoform 5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-12
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 32
Unit cell lengths	73.964, 73.964, 67.207
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.912 - 1.948
R-factor	0.1842
Rwork	0.182
R-free	0.22340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dmk
RMSD bond length	0.004
RMSD bond angle	0.869
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.948
Number of reflections	30014
<I/σ(I)>	17.7
Completeness [%]	99.8
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	289	50mM Calcium chloride dihydrate, 30%(w/v) polyethylene glycol monomethyl ether 550, 0.1M Bis-Tris PH6.5