4WUJ
Structural Biochemistry of a Fungal LOV Domain Photoreceptor Reveals an Evolutionarily Conserved Pathway Integrating Blue-Light and Oxidative Stress
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-25 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9179 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.342, 102.225, 71.550 |
| Unit cell angles | 90.00, 91.07, 90.00 |
Refinement procedure
| Resolution | 44.334 - 2.230 |
| R-factor | 0.2087 |
| Rwork | 0.206 |
| R-free | 0.26020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3d72 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.485 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.270 |
| High resolution limit [Å] | 2.230 | 6.050 | 2.230 |
| Rmerge | 0.047 | 0.044 | 0.118 |
| Total number of observations | 121511 | ||
| Number of reflections | 30153 | ||
| <I/σ(I)> | 25.6 | ||
| Completeness [%] | 96.1 | 84.3 | 78.3 |
| Redundancy | 4 | 3.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 100 mM Hepes pH 7.5 buffer, 6% (v/v) Peg400 and 1.4M ammonium sulfate |
