4WU5
HLA-A24 in complex with HIV-1 Nef134-8(wt)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-29 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 57.687, 67.070, 117.727 |
| Unit cell angles | 90.00, 99.01, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.400 |
| R-factor | 0.2333 |
| Rwork | 0.231 |
| R-free | 0.27090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vxn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.925 |
| Data reduction software | SCALA |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 35040 |
| <I/σ(I)> | 17.4 |
| Completeness [%] | 97.7 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 3350, 200 mM sodium nitrate |
