4WU4
Crystal structure of E. faecalis DNA binding domain LiaRD191N complexed with 22bp DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-01 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.336, 77.248, 104.987 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.740 - 2.300 |
R-factor | 0.214 |
Rwork | 0.208 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.602 |
Data scaling software | HKL-2000 |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.097 | 0.904 |
<I/σ(I)> | 26 | 2.667 |
Completeness [%] | 99.9 | 99.2 |
Redundancy | 13.7 | 11.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 283 | 0.03 M Bis-Tris propane /3.4, 0.07 M Citric acid, 18% PEG 3,350, 0.05% LDAO, 0.1 M Sodium malonate. |