4WTX
Crystal structure of the fourth FnIII domain of integrin beta4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-21 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 57.260, 57.260, 76.290 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.796 - 1.500 |
| R-factor | 0.1957 |
| Rwork | 0.195 |
| R-free | 0.21810 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.335 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELXDE |
| Refinement software | PHENIX (1839) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.800 | 1.540 | |
| High resolution limit [Å] | 1.500 | 6.710 | 1.500 |
| Rmerge | 0.061 | 0.019 | 1.406 |
| Rmeas | 0.062 | 0.019 | 1.433 |
| Total number of observations | 485023 | ||
| Number of reflections | 19975 | 290 | 1509 |
| <I/σ(I)> | 33.88 | 129.75 | 3.3 |
| Completeness [%] | 95.3 | 100 | 99.8 |
| Redundancy | 24.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 0.1 M Sodium Acetate (pH 4.2), 0.7 M NaH2PO4, 1.05 M K2HPO4, 2 mM DTT |
