4WTQ

Structure of the Ssl1 laccase mutant M295L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.900002
Spacegroup name	P 21 21 21
Unit cell lengths	51.528, 104.795, 164.389
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.580 - 1.800
R-factor	0.17277
Rwork	0.171
R-free	0.20812
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m3h
RMSD bond length	0.019
RMSD bond angle	1.859
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0071)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.960	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.070	0.508
Number of reflections	82580
<I/σ(I)>	15.3	4
Completeness [%]	99.1	99.3
Redundancy	6.3	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	50 mM HEPES, pH 7.0, 1.0-1.3 M ammonium sulfate, 5-20 mM hexamminecobalt(III) chloride