4WTP

Crystal structure of glycoside hydrolase family 17 beta-1,3-glucanosyltransferase from Rhizomucor miehei

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-24
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	45.887, 50.366, 98.405
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.490 - 1.300
R-factor	0.17849
Rwork	0.178
R-free	0.18640
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.385
Data scaling software	HKL-2000
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall
Low resolution limit [Å]	27.927
High resolution limit [Å]	1.299
Number of reflections	52655
<I/σ(I)>	71.5
Completeness [%]	92.4
Redundancy	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	25% (w/v) PEG 8000, 0.2M (NH4)2SO4