4WT5

The C-terminal domain of Rubisco Accumulation Factor 1 from Arabidopsis thaliana, crystal form II

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-05-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97856
Spacegroup name	P 21 21 21
Unit cell lengths	39.680, 60.789, 143.267
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.568
R-factor	0.2128
Rwork	0.210
R-free	0.27920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wt4
RMSD bond length	0.008
RMSD bond angle	1.272
Data scaling software	SCALA (3.3.16)
Phasing software	MOLREP (10.2.35)
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	71.633	47.756	2.710
High resolution limit [Å]	2.568	8.120	2.570
Rmerge		0.032	0.909
Rmeas	0.110
Rpim	0.051	0.018	0.477
Total number of observations	48923	1571	5727
Number of reflections	11314
<I/σ(I)>	11.9	30.8	2.1
Completeness [%]	96.7	98.1	81.8
Redundancy	4.3	3.7	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	200 mM KH2PO4 and 20% (w/v) PEG-5000MME