4WT4
The C-terminal domain of Rubisco Accumulation Factor 1 from Arabidopsis thaliana, crystal form I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-23 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.00004 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 157.538, 34.361, 106.885 |
| Unit cell angles | 90.00, 93.67, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.810 |
| R-factor | 0.2423 |
| Rwork | 0.240 |
| R-free | 0.28940 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.063 |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.509 | 45.509 | 2.960 |
| High resolution limit [Å] | 2.800 | 8.870 | 2.800 |
| Rmerge | 0.012 | 0.697 | |
| Rmeas | 0.052 | ||
| Rpim | 0.027 | 0.008 | 0.429 |
| Total number of observations | 52140 | 1759 | 7064 |
| Number of reflections | 14418 | ||
| <I/σ(I)> | 20 | 69 | 1.9 |
| Completeness [%] | 99.2 | 99.1 | 96.5 |
| Redundancy | 3.6 | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 10% PEG 3350 |
