4WT0
Crystal structure of the DNA binding domains of LiaRD191N from E. faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-01 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 31.049, 76.934, 76.920 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.793 - 1.800 |
| R-factor | 0.189 |
| Rwork | 0.182 |
| R-free | 0.24640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.931 |
| Data reduction software | HKL-2000 |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.510 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.065 | 0.669 |
| <I/σ(I)> | 1.889 | |
| Completeness [%] | 96.9 | 92.6 |
| Redundancy | 13.7 | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.6 | 283 | 0.1 M Tris pH 8.6, 0.2 M LiSO4, 20% w/v PEG 3.350, 0.05% Tween-20, 10% Glycerol, 10 mM Praseodymium (III) acetate hydrate |
