4WST
The crystal structure of hemagglutinin from A/Taiwan/1/2013 influenza virus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-22 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 226.630, 100.612, 175.477 |
Unit cell angles | 90.00, 99.66, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.400 |
R-factor | 0.22336 |
Rwork | 0.222 |
R-free | 0.25264 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.676 |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.400 |
Number of reflections | 146479 |
<I/σ(I)> | 17.7 |
Completeness [%] | 96.6 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 8.5 | 293 | 0.01M nickel chloride, 0.1 M Tris-HCl, and 20% (w/v) PEG 2000MME |