4WSM
Complex of 70S ribosome with tRNA-Leu and mRNA with G-U mismatch in the first position in the A- and P-sites
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 209.500, 449.900, 618.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 153.310 - 3.300 |
| R-factor | 0.2313 |
| Rwork | 0.185 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3TVE 3TVF |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 200.000 | 3.400 | |
| High resolution limit [Å] | 3.300 | 100.000 | 3.300 |
| Rmerge | 0.537 | 0.136 | 5.001 |
| Rmeas | 0.541 | 0.147 | 5.049 |
| Total number of observations | 50295385 | ||
| Number of reflections | 867119 | 11 | 73835 |
| <I/σ(I)> | 9.1 | 12.83 | 1.51 |
| Completeness [%] | 99.9 | 23.9 | 100 |
| Redundancy | 58 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | Tris-acetate, KSCN, PEG 550MME, PEG 20K |
