4WSB
Bat Influenza A polymerase with bound vRNA promoter
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-07-20 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9763 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 268.190, 149.320, 88.620 |
Unit cell angles | 90.00, 98.00, 90.00 |
Refinement procedure
Resolution | 19.996 - 2.650 |
R-factor | 0.2179 |
Rwork | 0.215 |
R-free | 0.26710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wsa |
RMSD bond length | 0.004 |
RMSD bond angle | 0.732 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: dev_1760)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.720 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.082 | 1.439 |
Number of reflections | 99462 | |
<I/σ(I)> | 10.9 | 1.14 |
Completeness [%] | 99.2 | 96 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 281 | Bat influenza polymerase protein in 50 mM HEPES-NaOH, 500 mM NaCl, 5 % glycerol, 2 mM TCEP, pH = 7.5 was adjusted to a concentration of 10 mg per ml, mixed in a 1:1 ratio with vRNA, which was an equimolar mixture of nucleotides 1-16 from the 5 prime end and nucleotides 1-18 or 3-18 from the 3 prime end. Protein-RNA was mixed with mother liquor containing 0.7-1.5 M sodium-potassium phosphate at pH 5.0 |