4WRQ

Crystal Structure of 14-3-3 zeta with Chibby peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-08-09
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	70.830, 71.960, 130.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.078 - 2.409
R-factor	0.2073
Rwork	0.203
R-free	0.25640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qja
RMSD bond length	0.008
RMSD bond angle	1.123
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	65.490	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.072	0.516
Number of reflections	26474
<I/σ(I)>	13.9
Completeness [%]	98.7	89.5
Redundancy	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	16-20% PEG 3350, ammonium formate