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4WRA

Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin.

This is a non-PDB format compatible entry.
Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSLS BEAMLINE X06SA
Synchrotron siteSLS
BeamlineX06SA
Temperature [K]100
Detector technologyPIXEL
Collection date2013-06-07
DetectorDECTRIS PILATUS 6M
Wavelength(s)1.000
Spacegroup nameP 21 21 21
Unit cell lengths209.900, 450.900, 622.700
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution225.450 - 3.050
Rwork0.195
R-free0.24890
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)3TVE 3TVF
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwarePHASER
Refinement softwarePHENIX
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]300.0003.150
High resolution limit [Å]3.050125.0003.050
Rmerge0.3820.0344.278
Rmeas0.3880.0384.406
Total number of observations34273476
Number of reflections11081587101521
<I/σ(I)>9.5714.460.91
Completeness [%]99.929.299.9
Redundancy30.9
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP7298Tris-acetate, KSCN, PEG 550MME, PEG 20K

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