4WRA
Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 209.900, 450.900, 622.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 225.450 - 3.050 |
Rwork | 0.195 |
R-free | 0.24890 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3TVE 3TVF |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 300.000 | 3.150 | |
High resolution limit [Å] | 3.050 | 125.000 | 3.050 |
Rmerge | 0.382 | 0.034 | 4.278 |
Rmeas | 0.388 | 0.038 | 4.406 |
Total number of observations | 34273476 | ||
Number of reflections | 1108158 | 7 | 101521 |
<I/σ(I)> | 9.57 | 14.46 | 0.91 |
Completeness [%] | 99.9 | 29.2 | 99.9 |
Redundancy | 30.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | Tris-acetate, KSCN, PEG 550MME, PEG 20K |