4WRA

Complex of 70S ribosome with tRNA-Tyr and mRNA with A-A mismatch in the first position in the A-site and with antibiotic paromomycin.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	209.900, 450.900, 622.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	225.450 - 3.050
Rwork	0.195
R-free	0.24890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3TVE 3TVF
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	300.000		3.150
High resolution limit [Å]	3.050	125.000	3.050
Rmerge	0.382	0.034	4.278
Rmeas	0.388	0.038	4.406
Total number of observations	34273476
Number of reflections	1108158	7	101521
<I/σ(I)>	9.57	14.46	0.91
Completeness [%]	99.9	29.2	99.9
Redundancy	30.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	Tris-acetate, KSCN, PEG 550MME, PEG 20K