4WQU

Crystal structure of the Thermus thermophilus 70S ribosome in complex with elongation factor G trapped by the antibiotic dityromycin

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-06-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97890
Spacegroup name	P 21 21 21
Unit cell lengths	209.840, 450.580, 623.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.810 - 2.800
R-factor	0.212
Rwork	0.209
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.767
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.870
High resolution limit [Å]	2.800	2.800
Rmerge	0.128	1.179
Number of reflections	1353291
<I/σ(I)>	11.27	1.3
Completeness [%]	94.6	92
Redundancy	3.88

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.6-3.0% PEG-20K, 7-10% MPD, 0.5mM BME