4WQ0

Crystal structure of cytochrome P450 CYP107W1 from Streptomyces avermitilis in complex with Oligomycin A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-20
Detector	ADSC QUANTUM 270
Wavelength(s)	0.979
Spacegroup name	P 43 21 2
Unit cell lengths	127.812, 127.812, 76.584
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.420 - 2.700
R-factor	0.2191
Rwork	0.215
R-free	0.28850
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.915
Data reduction software	SCALEPACK
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.600
Number of reflections	19627
<I/σ(I)>	26.2
Completeness [%]	99.6
Redundancy	8.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	287	Isopropanol, Ammonium citrate, citric acid