4WPE
Crystal Structure of Hof1p F-BAR domain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-08-05 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97949 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 137.178, 43.893, 95.858 |
Unit cell angles | 90.00, 133.53, 90.00 |
Refinement procedure
Resolution | 39.908 - 2.700 |
R-factor | 0.2104 |
Rwork | 0.208 |
R-free | 0.24660 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.607 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.700 |
Number of reflections | 11489 |
<I/σ(I)> | 33.9 |
Completeness [%] | 98.9 |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 294 | 0.1 M Na citrate, pH 5.5, containing 0.1 M ammonium acetate, and 5-7% (w/v) PEG3350 |