4WPE

Crystal Structure of Hof1p F-BAR domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2007-08-05
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.97949
Spacegroup name	C 1 2 1
Unit cell lengths	137.178, 43.893, 95.858
Unit cell angles	90.00, 133.53, 90.00

Refinement procedure

Resolution	39.908 - 2.700
R-factor	0.2104
Rwork	0.208
R-free	0.24660
RMSD bond length	0.003
RMSD bond angle	0.607
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.700
Number of reflections	11489
<I/σ(I)>	33.9
Completeness [%]	98.9
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	294	0.1 M Na citrate, pH 5.5, containing 0.1 M ammonium acetate, and 5-7% (w/v) PEG3350