4WPC
Crystal structure of Rgd1p F-BAR domain in complex with inositol phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-09 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.91790 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 178.993, 74.110, 105.734 |
| Unit cell angles | 90.00, 104.27, 90.00 |
Refinement procedure
| Resolution | 30.773 - 3.340 |
| R-factor | 0.2632 |
| Rwork | 0.261 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wpe |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.115 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.300 |
| Number of reflections | 19341 |
| <I/σ(I)> | 14 |
| Completeness [%] | 99.7 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.1 M citrate, pH 5.5, containing 0.1-0.2 M (NH4)2SO4 plus 10-20% (w/v) PEG3350 |
