4WPB

Vascular endothelial growth factor in complex with alpha/beta-VEGF-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-19
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	39.100, 78.400, 56.500
Unit cell angles	90.00, 102.80, 90.00

Refinement procedure

Resolution	45.078 - 3.110
R-factor	0.2454
Rwork	0.240
R-free	0.29830
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.025
RMSD bond angle	1.475
Data reduction software	XDS (November 11, 2013)
Data scaling software	XSCALE (January 10, 2014)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9-1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.080		3.180
High resolution limit [Å]	3.100	13.870	3.100
Rmerge	0.083	0.037	0.243
Rmeas	0.097	0.045	0.283
Total number of observations	22316
Number of reflections	6096	70	447
<I/σ(I)>	14.26	20.54	7.45
Completeness [%]	99.6	95.9	100
Redundancy	3.7		3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M magnesium formate dihydrate, 15% (w/v) PEG3350, 30% (v/v) glycerol