4WOY
Crystal structure and functional analysis of MiD49, a receptor for the mitochondrial fission protein Drp1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-23 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.99 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 100.674, 153.390, 109.015 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 36.980 - 2.400 |
R-factor | 0.2067 |
Rwork | 0.206 |
R-free | 0.24290 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.355 |
Refinement software | PHENIX ((phenix.refine: dev_1760)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.980 | 2.480 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.051 | 0.694 |
Number of reflections | 32951 | |
<I/σ(I)> | 21.5 | 2.1 |
Completeness [%] | 98.9 | 99.51 |
Redundancy | 4.3 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 100 mM HEPES (pH 7.0), 20 mM MgCl2, 20% polyacrylic acid 5,100 (w/v) |