4WOR
Staphylococcal nuclease in complex with Ca2+ and thymidine-3'-5'-diphosphate (pdTp) at room temperature
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE A1 |
Synchrotron site | CHESS |
Beamline | A1 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 1995-02-17 |
Detector | PRINCETON 2K |
Wavelength(s) | 0.908 |
Spacegroup name | P 41 |
Unit cell lengths | 48.499, 48.499, 63.430 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.249 - 1.596 |
R-factor | 0.1811 |
Rwork | 0.180 |
R-free | 0.20420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1snc |
RMSD bond length | 0.017 |
RMSD bond angle | 1.284 |
Refinement software | PHENIX ((phenix.refine: dev_1779)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.250 | 1.653 |
High resolution limit [Å] | 1.596 | 1.596 |
Rmerge | 0.076 | 0.537 |
Number of reflections | 19093 | |
<I/σ(I)> | 8.22 | 1.55 |
Completeness [%] | 97.6 | 96.06 |
Redundancy | 2 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.15 | 277 | 2-methyl-2,4-pentanediol (MPD), potassium phosphate, thymidine-3',5'-diphosphate (pdTp), Ca2+ |